Transcortin: a corticosteroid-binding protein of plasma. III. The effects of various steroids.

Plasma protein binding of triamcinolone-H3 and hydrocortisone-4-C14.

It has become increasingly apparent in recent years that certain structural alterations of corticosteroids cause an increased biological potency of the resultant steroid (2). A number of chemical and microbiological alteration products of hydrocortisone exhibit enhanced anti-inflammatory potency coupled with unchanged or depressed mineralocorticoid activity (2, 3). As a result, steroids bearing...

Plasma Protein Binding of Triamcinolone-H3 and Hydrocortisone4C4*

It has become increasingly apparent in recent years that certain structural alterations of corticosteroids cause an increased biological potency of the resultant steroid (2). A number of chemical and microbiological alteration products of hydrocortisone exhibit enhanced anti-inflammatory potency coupled with unchanged or depressed mineralocorticoid activity (2, 3). As a result, steroids bearing...

Transcortin: a corticosteroid-binding protein of plasma. II. Levels in various conditions and the effects of estrogens.

A combined adsorption-gel filtration technique for the determination of the cortisol-binding capacity of transcortin.

A combined adsorption-gel filtration technique has been developed for the quantitation of the cortisol-binding capacity of transcortin: Endogenous steroids are removed from plasma by adsorption on uncoated charcoal. Saturation of the "stripped" binding sites of transcortin is accomplished by equilibrating the sample with a definite amount of labeled cortisol of low specific activity (0.1 muCi/m...

Transcortin: a corticosteroid-binding protein of plasma. V. In vitro inhibition of cortisol metabolism.